Quantitative RNA Techniques

The isolation of RNA is a central, though unappreciated method in molecular biology. Careful planning and attention to experimental methods are absolutely necessary for the isolation of biologically and chemically stable RNA which reflects the cellular biochemistry. This laboratory-intensive workshop emphasizes methods which favor the isolation of high quality RNA, coupled with numerous contemporary and classical techniques for quantifying transcriptional activity. Continuing Education Units (CEUs) available.

This workshop is conducted by Dr. Robert Farrell, author of RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 4th ed. (Elsevier Academic Press, 2010).


Topics

         Why Study RNA?

         Planning an RNA Extraction

         The Ribonuclease Peril

         Isolation of Total Cellular RNA

         Isolation of Cytoplasmic RNA

         Northern Analysis

         Nuclease Protection Assay

         Selection of Poly(A)+ RNA by Magnetic Bead Technology

         RNA Enrichment and cDNA Synthesis Strategies

         RT-PCR, Including Long-Range PCR Applications

         Overview of Quantitative Approaches, Including Real-Time PCR

         Introduction to RNA Interference (RNAi)

         5- and 3- RACE

         Non-isotopic Probe Labeling and Detection by Chemiluminescence

         mRNA Differential Display (Lecture only)

         Nuclear Runoff Assay (Lecture only)

 

Date / Location / Tuition

Delivered on-site at your location in 2011. Please contact us for details.

REGISTER


Home

Exon-Intron, Inc.
P.O. Box 395
Loganville, PA 17342
Tel: 717-428-0270
Fax: 717-771-4062
workshop@DNAtech.com