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Quantitative RNA Techniques The isolation of RNA is a central, though unappreciated method in molecular biology. Careful planning and attention to experimental methods are absolutely necessary for the isolation of biologically and chemically stable RNA which reflects the cellular biochemistry. This laboratory-intensive workshop emphasizes methods which favor the isolation of high quality RNA, coupled with numerous contemporary and classical techniques for quantifying transcriptional activity. Continuing Education Units (CEUs) available.
► Why Study RNA? ► Planning an RNA Extraction ► The Ribonuclease Peril ► Isolation of Total Cellular RNA ► Isolation of Cytoplasmic RNA ► Northern Analysis ► Nuclease Protection Assay ► Selection of Poly(A)+ RNA by Magnetic Bead Technology ► RNA Enrichment and cDNA Synthesis Strategies ► RT-PCR, Including Long-Range PCR Applications ► Overview of Quantitative Approaches, Including Real-Time PCR ► Introduction to RNA Interference (RNAi) ► 5- and 3- RACE ► Non-isotopic Probe Labeling and Detection by Chemiluminescence ► mRNA Differential Display (Lecture only) ► Nuclear Runoff Assay (Lecture only) Date / Location / Tuition Delivered on-site at your location in 2011. Please contact us for details. Exon-Intron, Inc. |