|
|
DNA
Typing Slight but unique
differences in the banding pattern of DNA fragments from different
individuals of a species are observed when subjected to restriction enzyme analysis.
These variations in the DNA are called restriction fragment length
polymorphisms (RFLPs) and are used as markers on both physical and genetic
linkage maps. Such differences in the RFLP profiles have revolutionized
criminal investigations and have become powerful tools in the identification
of individuals in paternity and maternity cases, population genetics, and in
the diagnosis of a variety of diseases. More recently,
polymerase chain reaction (PCR)-based tests have been used to obtain a DNA
profile. Such tests are generally less involved, providing rapid results that
can serve as an alternative or as a complement to RFLP tests. This integrated
laboratory and lecture course is designed to provide the participant with a
basic understanding of the principles and methodologies used in RFLP and
PCR-based analyses. The practical applications of RFLP analysis, coupled with
the use of PCR-amplified products the VNTR locus D1S80, are emphasized in
selected topics for the forensic and research scientist as well as for those
in the legal community. Continuing Education Units (CEUs) available.
► Understanding RFLP, VNTR, and Microsatellite Organization ►
Purification and Preparation of DNA for RFLP Analysis ►
Gel Electrophoresis and Blotting ►
Hybridization Procedures ►
PCR Fingerprinting of the VNTR D1S80 Locus ►
Non-Isotopic Probe Synthesis and Detection by Chemiluminescence ►
Random Amplified Polymorphic DNA Analysis Date
/ Location / Tuition This workshop will be conducted on-site only (at your
location) in 2010, and is presented in conjunction with DNA Diagnostics, Inc. Please
contact us for details. Exon-Intron, Inc. |